|
KMID : 1101320040360010001
|
|
Korean Journal of Clinical Laboratory Science
2004 Volume.36 No. 1 p.1 ~ p.6
|
|
|
Molecular Typing of Vibrio parahaemolyticus by Repetitive Element-PCR (rep-PCR)
|
|
Kim Won-Sik
Hong Seung-Bok
Lee Kyung
Lee Jung-Nam
Shin Kyeong-Seob
|
|
|
Abstract
|
|
|
|
The enterobacterial repetitive intergenic consensus (ERIC)-PCR is a recently described DNA fingerprinting technique based on amplification of repetitive element distributed in bacteria. We applied of ERIC-PCR to clinical isolates of Vibrio parahaemolyticus and other bacteria associated diarrhea. Twenty isolates of V. parahaemolyticus were used for intragenic genotyping, which were isolated from 2001 to 2002 in Chungbuk National University hospital. For interspecies genotyping, V. vulnificus, V. alginolyticus, V. parahaemolyticus, Escherichia coli, Salmonella and Shigella spp. were used. The genotyping were analyzed by ERIC-PCR. The genotyping of V. parahaemolyticus were grouped two major pattern (A, B) and were subdivided into 10 subtypes (A1, A2, B1-B8) by ERIC-PCR. These method distinctly differentiated bacterial species associated diarrhea. Those results show that ERIC-PCR can be reliable and efficient method for genotyping of V. parahaemolyticus and bacteria associated diarrhea.
|
|
|
KEYWORD
|
|
|
Vibrio parahaemolyticus, Genotyping, ERIC-PCR, Diarrhea
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|